Description
Derived from a breast cancer biopsy removed from a 69 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade II, 90% of tumour cells stained positive for oestrogen receptors and cells were negative for progesterone receptor staining. The VP303 cell line derived from the tumour did not stain positive for oestrogen receptors. The individual was recorded to be alive 5 years after surgery performed to remove the tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.
Adherent Suspension
Adherent
Suggested Medium
MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.
Quality Control
VP303 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
VP303 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
VP303 Cell Line is for research use only
