Description
Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) , also known as CDw150, which cell line is a receptor for measles virus. Clones of transfected cells were selected in the presence of geneticin (also known as G418). Vero/hSLAM cells can be used to isolate measles virus from human clinical samples and are a substitute for the B95a cell line for this purpose. Mumps susceptibility has also been demonstrated at the depositor's laboratory.
Adherent Suspension
Adherent
Suggested Medium
MEM with Earle's salts + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) + 0.4mg/ml Geneticin. It is essential that geneticin is added to the culture medium as it is required to maintain the expression of the SLAM gene.
Quality Control
Vero/hSLAM Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
Vero/hSLAM Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Vero/hSLAM Cell Line is for research use only
