Description
Control cell line used for the establishment of mouse homo mutant ES cell lines.
Suggested Medium
Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells.
Quality Control
vdR2-4 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Subculture
0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish
Shippment
vdR2-4 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
vdR2-4 Cell Line products are for research use only.
