Description
Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two or three copies per cell.
Adherent Suspension
Adherent
Suggested Medium
EMEM EBSS + 2mM Glutamine + 0.1mM Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) + 1.5g/L sodium bicarbonate + 1.0mM sodium pyruvate.
Quality Control
UM-UC-3 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
UM-UC-3 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
UM-UC-3 Cell Line is for research use only
