Description
UKRV-Mel-6b (ESTDAB-222) was established from the cutaneous/subcutaneous metastasis in a 51 year-old female patient with metastatic melanoma (primary nodular melanoma stage IV). The UKRV-Mel-6b cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission's fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB (http//www.ebi.ac.uk/ipd/estdab) contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. This information is appended below. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation. Robinson J, Waller MJ, Fail SC, Marsh SG. 2006 The IMGT/HLA and IPD databases. Hum Mutat 27(12)11929.PMID 16944494. Pawelec G, Marsh SG. 2006 ESTDAB a collection of immunologically characterised melanoma cell lines and searchable databank. Cancer. Immunol Immunother. 55(6)623-7.PMID 16421722. Robinson J, Roberts CH, Dodi IA, Madrigal JA, Pawelec G, Wedel L, Marsh SG.2009 The European searchable tumour line database. Cancer. Immunol Immunother. 58(9)1501-6. PMID 19172270.
Quality Control
Cell lines were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
