Description
A human glioblastoma astrocytoma derived from a malignant tumour by explant technique. This catalogue entry represents a new deposit of U-373 MG obtained from the originator's laboratory in Uppsala; it has been designated U-373 MG (Uppsala) to distinguish it from U-373 MG (ECACC catalogue number 89081403) which is now known to be identical, by Short Tandem Repeat (STR)-PCR profiling, to another glioblastoma cell line, U-251 MG (please read below for more information about this discovery). U-373 MG (Uppsala) has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. Background to the identity query for the cell line U-373 MG The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator's laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under catalogue number 89081403 has been re-named as U-251 (formerly known as U-373 MG).
Adherent Suspension
Adherent
Suggested Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Quality Control
Cell lines were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Pleomorphic/astrocytoid
Shippment
The cells are shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Our cell products are for research use only. They are not authorized for human, in vitro diagnostic procedures or for therapeutic procedures.
