Description
Immortalized human hepatic stellate cell line (established from LI90).
Suggested Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum.
Quality Control
TWNT-1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
epithelial-like
Subculture
0.05% trypsin and 0.53 mM EDTA. split ratio = 1/3 - 1/4. Optimal seeding density 1.5 - 6.0 x 10^4 cells/cm2
Shippment
TWNT-1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
TWNT-1 Cell Line products are for research use only.
