Description
Rat embryo cell line with ts mutation for cell cycle progression from G1 to S phase.
Suggested Medium
Dulbecco's modified Eagle's medium with 0.1 % glucose and 10% fetal calf serum.
Quality Control
tsJT663 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
epithelial-like
Subculture
Cells are harvested after treatment with 0.125% trypsin and 0.01% EDTA. Gentle piptting may be reauired to detach cells from dishes. Subculture once a week at split ratio=1/8 at 34 C.
Shippment
tsJT663 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
tsJT663 Cell Line products are for research use only.