Description
This cell line is a conditionally immortalised dendritic cell line which was established from bone marrow of CBA mice transgenic for a thermo labile mutant of the SV40 large T antigen under the control of the Class I Kb promoter. At 33-37°C it divides in the absence of GM-CSF. It shares a number of cell surface markers with bone marrow macrophages, but unlike macrophages is constitutively MHC Class II+. Transfer to 39°C, arrests growth and results in up-regulation of surface markers such as B7.1, CD40 and intercellular adhesion molecule-1. Mycoplasma eradicated prior to deposit at Glow Biologics.
Adherent Suspension
Semi-adherent
Suggested Medium
Iscove's MEM + 5% Foetal Bovine Serum (FBS) + 2 mM Glutamine + 0.05 mM 2Mercaptoethanol (2ME).
Quality Control
tsDC Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
tsDC Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
tsDC Cell Line is for research use only
