Description
Trex1 KO cell line is a spontaneously transformed mouse embryonic fibroblast (MEF) cell line. The line was derived from Trex1-/- knockout mouse by repeated passaging in culture using standard techniques for culturing primary MEFs. Trex1 is the major 3' DNA exonuclease in mammalian cells, and mutations in the human TREX1 gene can cause Aicardi-Goutières syndrome, characterized by perturbed immunity. Trex1-deficient cells show defective G1/S transition, with increased cell doubling time and chronic ATM-dependent checkpoint activation, even in the absence of exogenous stress, correlating with persistent single-stranded DNA molecules produced in S phase, which accumulate in the ER. Trex1 KO can be used to study novel interconnections between DNA replication, DNA damage checkpoint signalling and antiviral-like autoimmune responses.
Adherent Suspension
Adherent
Suggested Medium
DMEMHAMS F12 (31) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
Trex1 KO Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
Trex1 KO Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Trex1 KO Cell Line is for research use only
