Description
The cell line TRA-171 has been established from freshly hatched larvae of Toxorhynchites amboinensis. After eggs had been surface-sterilised, they were allowed to hatch in MM / VP12 medium and were mechanically damaged at the anterior or posterior ends of the body in 0.25% trypsin. After washing and cultivation at 22 - 25°C small patches of cells were visible leading to the isolation of the cell line TRA-171 after 72 days in culture. The cells have been adapted to grow in a 11 mixture of Leibovitz L-15 and Mitsuhashi and Maramorosch media plus supplements. Four serotypes of laboratory adapted and three type strains (1, 2 and 3) of un-adapted dengue virus will replicate in the cells at 28°C.
Adherent Suspension
Adherent
Suggested Medium
L15 Mitsuhashi and Maramorosch Insect Medium (11) + 2mM Glutamine + 0.05% BSA + 1% NEAA + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS)
Quality Control
TRA-171 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Fibroblast-like
Shippment
TRA-171 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
TRA-171 Cell Line is for research use only
