Description
Peripheral blood lymphocytes from a healthy female Tunisian immortalised by EBV transformation. The cell line contains the following deletions, in the human immunoglobulin heavy locus (IGH on chromosome 14 at 14q32.33), of 6 and 3 IGHC genes, respectively on one chromosome 14, deletion I (del G1-EP1-A1-GP-G2-G4) and on the other chromosome 14, deletion II (del EP1-A1-GP). The Gm-Am genotype is Gm5*; - ; - A2m2/Gm21*;17,1; .. A2m2 (Gmb*; - ; - A2m2/Gmg*;za;.. A2m2). Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). Only one allotype is defined on the gamma 2 chain and may or may not be present; (..) indicates the absence of this allotype. An Epstein-Barr-transformed lymphoblastoid cell line is available, from one son with identical deletions I and II, as ‘MOH (TOU II-3)’ (Catalogue No. 93093002) and, from one son homozygous for deletion I, as ‘EZZ (TOU II-4)’ (Catalogue No. 93093003).
Adherent Suspension
Suspension
Suggested Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
Cell lines were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
The cells are shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Our cell products are for research use only. They are not authorized for human, in vitro diagnostic procedures or for therapeutic procedures.
