Description
Immortalized human-liver endothelial cell line with SV40T and hTERT. (Note recommended medium is very specific.)
Suggested Medium
Mochitate modified MCDB-131 medium with 50% of EGM-2 SingleQuot Kit (clontics #CC-4176; ascorbic acid was substituted by ascorbic acid phosphate magnesium salt n-Hydrate (0.2 mM))
Quality Control
TMNK-1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
epithelial-like
Subculture
Cells were harvested after treatment with accutase (Innovative cell technologies) and split ratio=1/3-1/4
Shippment
TMNK-1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
TMNK-1 Cell Line products are for research use only.
