Description
293T cells serially transfected with synthetic HIV Gag-Pol sequences expressed from a Murine Leukaemia Virus (MLV) vector; HIV tat sequences expressed from a MLV vector; HIV rev sequence expressed from a MLV vector, and RD114 envelope protein (with HIV Protease Cleavage Site) expressed from a plasmid. Finally, Green Fluorescent Protein (GFP) introduced by infection with a HIV expression vector. These cells stably express synthetic HIV Gag-Pol, HIV tat and HIV rev sequences, RD114 envelope protein with HIV Protease Cleavage Site and a GFP expressing HIV vector. Therefore, these cells produce disabled lentivirus containing the GFP gene for the efficient transduction of dividing and non-dividing cells. Genetically Modified Organism Class II.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
STAR-RDpro-HV#1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
STAR-RDpro-HV#1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
STAR-RDpro-HV#1 Cell Line is for research use only
