Description
Sp2/0-Ag14 cell line adapted to grow in animal-component free, protein-free, chemically defined, serum-free medium (EX-CELL® Sp2/0 Serum-Free Medium for Sp2/0 Cells Chemically Defined, Sigma cat no 14660C). Sp2/0-Ag14 cell line is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. Can be used as a fusion partner for generating hybridomas. This cell line was previously known as SP2/0-Ag14 (AC-Free) however the name has been changed to Sp2/0-Ag14 (serum-free) to make the serum free status of this culture more obvious to users.
Adherent Suspension
Suspension
Suggested Medium
EX-CELL Sp2/0 Serum-Free Medium (Sigma cat no. 14660C) + 8mM Glutamine. When freezing cells down use 5050 fresh culture mediumconditioned medium plus 10% DMSO)
Quality Control
Sp2/0-Ag14 (serum-free) Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
Sp2/0-Ag14 (serum-free) Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Sp2/0-Ag14 (serum-free) Cell Line is for research use only
