Description
a GM-CSF dependent cell line with an t8,21 chromosome translocation estabulished from a patient attacked by acute myeloid leukemia(AML)
Suggested Medium
RPMI 1640 with 10% Fetal Bovine Serum and 10 ng/ml rhGM-CFS(recombinant human granurocyte-macrophage colonystimulating factor)
Quality Control
SKNO-1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Subculture
Harvest cells after treatment with 0.01% EDTA and 0.125% trypsin. Subculture every two days and disperse cells completely before scoring cell number.
Shippment
SKNO-1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
SKNO-1 Cell Line products are for research use only.