Description
SH-SY5Y cell line is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH. SH-SY-5Y has dopamine-beta-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as neurite expression, noradrenalin uptake or neuronal markers routinely. Typically Glow Biologics SH-SY5Y cells are supplied at passage 12.
Adherent Suspension
Adherent
Suggested Medium
Ham's F12EMEM (EBSS) (11) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Quality Control
SH-SY5Y Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
SH-SY5Y Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
SH-SY5Y Cell Line is for research use only
