Description
This cell line was derived from neonatal Wistar rat Schwann cells being cultured for several months with intermittent exposure to the mitogen, cholera toxin and infrequent passaging to avoid premature transformation. After cloning one of these cultures by limiting dilution, the line SCL4.1/F7 was established growing without mitrogen. The cells display a subratum adhesion which is calcium-dependent in the millimolar range and pronounced contact-inhibition of growth. Confluent or subconfluent cultures readily cease proliferation and change to a differentiated morphology secreting an autocrine antiproliferative factor. Cells have been eradicated from mycoplasma at Glow Biologics. With increasing passage cells have been shown to undergo transformation. It is recommended to growth-arrest cells for a few days before subculture to avoid rapid transformation.
Adherent Suspension
Adherent
Suggested Medium
Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
SCL 4.1/F7 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Flattened or crescent shaped
Shippment
SCL 4.1/F7 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
SCL 4.1/F7 Cell Line is for research use only
