Description
The clonal cell line, RN46A-B14, was isolated following transfection of the gene encoding rat brain-derived neurotrophic factor into RN46A cells. RN46A-B14 cells synthesize and secrete biologically active BDNF in vitro and synthesize serotonin following partial membrane depolarization. Two weeks following RN46A-B14 cell transplantation into the adult rat cortex and hippocampus, there cell line is a threefold increase in survival of RN46A-B14 cells compared to RN46A cells. The grafted RN46A-B14 cells immunohistochemically stain for BDNF and 5-HT, while RN46A cells transfected with vector only are negative for both BDNF and 5HT. In addition, RN46A-B14 cells attain more morphologically complex phenotypes, indicating enhanced neuronal differentiation. Autocrine secretion of BDNF by RN46A-B14 cells thus potentiates survival and can be used to deliver both BDNF and 5-HT in vivo.
Adherent Suspension
Adherent
Suggested Medium
DMEM Ham's F12 (11) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.25mg/ml Geneticin (G418) + 0.1mg/ml hygromycin. Alternatively CNS medium can be used (see Kawamoto & Barrett 1986).
Quality Control
RN46A-B14 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Embryonic rat (day 13) medullary raphe
Morphology
Fibroblast morphology while proliferating and neuronal on differentiation
Shippment
RN46A-B14 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
RN46A-B14 Cell Line is for research use only
