Description
Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.
Alias
5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE
Endotoxin Testing
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity
Greater than 90% as determined by reducing SDS-PAGE.
Activity
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection KitThe specific activity is greater than 15000 pmol/min/µg.
Formulation
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
Storage
Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.
Warranty
For Research Use Only. Our recombinant proteins can not be used in diagnostic procedures.
