Description
Recombinant 2019-nCOV Spike Protein (S1+S2 ECD) was produced in Baculovirus-Insect Cells and fused with a His tag at the C-terminus.
Alias
S protein S1+S2; 2019-nCoV S1+S2 protein; SARS-COV2 S1+S2 protein; COVID-19 spike S1+S2 protein
Source
Baculovirus-Insect Cells
Molecular Mass
The recombinant 2019-nCoV Spike Protein (S1+S2 ECD, His tag) consists of 1209 a.a and predicts a molecular weight of 134.36 kDa.
Endotoxin Testing
<1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
The 2019-nCoV Spike Protein (S1+S2 ECD, His tag) can bind with Human ACE2 in functional ELISA assay.
Formulation
Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Storage
Store the 2019-nCoV Spike Protein (S1+S2 ECD, His tag) under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Warranty
For Research Use Only
