Description
The receptor binding domain (RBD) of Recombinant SARS-COV2 spike protein was produced in HEK293 cells and fused with the Fc region of mouse IgG1 at the C-terminus.
Alias
S protein RBD; 2019-nCoV S protein RBD; SARS-COV2 S protein; COVID-19 spike protein
Molecular Mass
The recombinant receptor binding domain (RBD) of 2019-nCoV spike consists of 457 amino acids and predicts a molecular weight of 51.5 kDa.
Endotoxin Testing
<1.0 EU per μg protein as determined by the LAL method.
Purity
> 95% as determined by SDS-PAGE.
Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Storage
Store the 2019-nCoV Spike Protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Warranty
For Research Use Only
