Description
The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.
Adherent Suspension
Adherent
Suggested Medium
DMEM, 2mM Glutamine, 10% FCS, 0.1% Epidermal growth factor, 1% insulin/transferrin/selenium mixture
Quality Control
RCP Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
RCP Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
RCP Cell Line is for research use only
