Description
Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL. The plasmid pcDNA3-VHL confers neomycin resistance and encodes the von Hippel-Lindau (VHL) tumour suppressor gene product pVHL. The original renal carcinoma cell line RCC4 is VHL-deficient. The RCC4 plus vector alone cell line can serve as a negative control cell line to study the effect of pVHL expression from pcDNA3-VHL. These cells are less tumourigenic than corresponding cell lines lacking pVHL function.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml
Quality Control
RCC4plusVHL Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
RCC4plusVHL Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
RCC4plusVHL Cell Line is for research use only
