Description
RCC BA85#21 (ELTHEM) cell line is a renal tumour cell line which was established from a primary tumour in a 62 year-old male patient with a clear cell carcinoma. The tumour tissue of the primary lesion was composed principally of clear cells with alveolar/tubular arrangement. The immunogenicity of the RCC BA85#21 (ELTHEM) cell line has been experimentally proven by in vitro stimulation under autologous and allogeneic conditions using dentritic cells loaded with cell lysate, cytotoxic assay and by the Elispot technique. Characterisation of RCC BA85#21 (ELTHEM) by phenotypic and molecular assays such as cytochemistry, flow cytometry, microarray analysis Real-Time PCR microsatellite instability and loss of heterozygosity is described.
Suggested Medium
RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Quality Control
RCC BA85#21 (ELTHEM) Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
RCC BA85#21 (ELTHEM) Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
RCC BA85#21 (ELTHEM) Cell Line is for research use only
