Description
The R74 line was derived by co-cultivation of 18 day-old Lewis rat embryonic fibroblasts with cells of a chicken positive for Rous sarcoma virus-33 (RSV-33). This cell population is clearly distinguishable from RT1 and R9 as it is characterised by the presence of fibroblastoid cells that tend to round up and form clusters of refractile cells. R74 cell line is also tumourigenic in chickens and rats.
Adherent Suspension
Adherent
Suggested Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).
Quality Control
R74 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Fibroblast-like
Shippment
R74 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
R74 Cell Line is for research use only
