Description
Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.
Adherent Suspension
Adherent
Suggested Medium
RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).
Quality Control
PNT1A Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
PNT1A Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
PNT1A Cell Line is for research use only
