Description
Normal keratinocyte cells immortalized with HPV16 virus genome and the growth is dependent to EGF. More than 95% of cells are diploid.
Suggested Medium
MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. MCDB153 with < 0.5% FCS was also used in original report.
Quality Control
PHK16-0b Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Subculture
Cells are harvested with 0.1% trypsin and 0.01% EDTA. Medium change every 2 days and subculture every 10 days.
Shippment
PHK16-0b Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
PHK16-0b Cell Line products are for research use only.
