Description
PG13 cells were derived from TK-NIH/3T3 cells. This retrovirus packaging cell line is based on the gibbon ape Leukaemia Virus (GaLV). Introduction of retroviral vectors results in the production of retrovirus virions capable of infecting cells from many species excluding mice. Introduced DNA may be lost after long-term passage. Selection against loss can be performed by growing the cells in medium with dialysed FBS and100nM amethopterin for 5 days followed by cultivation in HAT and untreated FBS for 5 days. Maintain cells for 4 days in HT medium to avoid toxic effects of residual amethopterin. Virus production has not been observed, but there is the risk that cells produce a virus similar to GaLV. The cell line has been tested positive for Reverse Transcriptase.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
PG13 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
PG13 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
PG13 Cell Line is for research use only
