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PEO1-CDDP Cell Line

Cat.No.
GBTC-2920E
Description
The PEO1-CDDP cell line was established by in vitro exposure of the PEO1 line to cisplatin commencing at 25 nMol/L cisplatin and increasing to one µMol/L  over a period of four months. The resistant cell line thus obtained was then passaged in the absence of cisplatin for a further four months to ensure that the resistance remained stable. PEO1-CDDP is from the same patient as the PEO1, PEO4 and PEO6 cell lines. The PEO1-CDDP cell line cell line cell line is an in vitro model to understand the changes in EGF signalling after the onset of cisplatin resistance.
Biosafety Level
1
Adherent Suspension
Adherent
Size
1x10^6 cells/vial
Suggested Medium
RPMI 1640 containing 10% heat-inactivated FCS. Cells are maintained routinely at 37°C in a humidified atmosphere of 5% CO2/95% air. Supplement medium with Cisplatin (CDDP) at 1 µMol/L.
Quality Control
PEO1-CDDP Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Ovary
Morphology
Polygonal epithelia
Shippment
PEO1-CDDP Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
PEO1-CDDP Cell Line is for research use only

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