Description
The PEO1-CDDP cell line was established by in vitro exposure of the PEO1 line to cisplatin commencing at 25 nMol/L cisplatin and increasing to one µMol/L over a period of four months. The resistant cell line thus obtained was then passaged in the absence of cisplatin for a further four months to ensure that the resistance remained stable. PEO1-CDDP is from the same patient as the PEO1, PEO4 and PEO6 cell lines. The PEO1-CDDP cell line cell line cell line is an in vitro model to understand the changes in EGF signalling after the onset of cisplatin resistance.
Adherent Suspension
Adherent
Suggested Medium
RPMI 1640 containing 10% heat-inactivated FCS. Cells are maintained routinely at 37°C in a humidified atmosphere of 5% CO2/95% air. Supplement medium with Cisplatin (CDDP) at 1 µMol/L.
Quality Control
PEO1-CDDP Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polygonal epithelia
Shippment
PEO1-CDDP Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
PEO1-CDDP Cell Line is for research use only