Description
Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-alpha reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat-PCR analysis when tested at Glow Biologics. It cell line is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Adherent Suspension
Adherent
Suggested Medium
Coons Modified Hams F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS). OR Kaigns modified Hams F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).
Quality Control
PC-3 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
PC-3 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
PC-3 Cell Line is for research use only
