Description
P4E6 cell line cell line is an Immortalised human prostate cell line derived from a biopsy of a well-differentiated early stage prostate cancer) P4E6 was immortalised using human papillomavirus type E6 gene from PLXSN16E6 retroviral stock. The Y chromosome could not be detected in this cell line by short tandem repeat-PCR analysis when tested at Glow Biologics. It cell line is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Suggested Medium
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Quality Control
P4E6 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
P4E6 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
P4E6 Cell Line is for research use only
