Description
The P19 cell line was derived from an embryonal carcinoma induced in a C3H/He strain mouse. The cells can be cloned at high efficiency in medium containing 0.1mM beta-mercaptoethanol. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid. Aggregates of P19 cells differentiate into cardiac and skeletal muscle in the presence of dimethyl sulfoxide (DMSO). In the presence of both retinoic acid and DMSO cells develop as if exposed to retinoic acid only.
Adherent Suspension
Adherent
Suggested Medium
Alpha MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2.5% Foetal Bovine Serum (FBS) + 7.5% Calf Serum (CS).
Quality Control
P19 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
P19 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
P19 Cell Line is for research use only
