Description
The pluripotent human embryonal carcinoma cell line NTERA-2 clone D1, also known as NT2/D1, cell line is a subclone derived from the parent line NTERA-2. NTERA-2 cells were established from a nude mouse xenograft tumour of TERA-2 cells, which were originally isolated from a lung metastasis from a 22 year old patient with primary embryonal carcinoma of the testis. The subline NTERA-2 clone D1 differentiates into neuronal and other cell types in response to retinoic acid (RA) or hexamethylene bisacetamide (HMBA). Differentiated cells become permissive for human cytomegalovirus (HCMV) or human immunodeficiency virus (HIV). Differentiation with RA as inducer results in glycolipid changes, appearance of neurons and induction of Homeobox (HOX) gene clusters. When NTERA-2 clone D1 cells are passaged at high cell density EC-like cells predominate. However, when cultured at low density many large flat cells differing from typical EC cells are formed.
Adherent Suspension
Adherent
Suggested Medium
DMEM containing 4.5 g/L glucose + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should be cryopreserved in 95% FCS5% DMSO
Quality Control
NTERA-2 clone D1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Testis (lung metastasis)
Shippment
NTERA-2 clone D1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
NTERA-2 clone D1 Cell Line is for research use only
