Description
A mouse embryonic stem cell (ES) line (Nd) containing a novel fluorescent reporter (VNP) to investigate the temporal dynamics of Nanog expression in mouse and to dissect the lineage potential at different Nanog expression levels. This novel reporter ES cell line allows the accurate monitoring the dynamic expression of the transcription factor Nanog in the pluripotent state. The cell line will be a valuable tool to obtain quantitative measurements of global gene expression in pluripotent mouse ES cells in different states, allowing a detailed molecular mapping of the pluripotent state.
Adherent Suspension
Adherent
Suggested Medium
Culture cells in Glasgow Modified Eagles Medium with 10% FCS + 2mM L-glutamine, 2ng/ml Leukemia Inhibitory Factor (LIF) and 1mMolar 2-mercaptoethanol on gelatin-coated (0.1%) cell culture plastic.
Quality Control
Nd ES Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Small round cell growing in spherical colonies
Shippment
Nd ES Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Nd ES Cell Line is for research use only
