Description
Normal ecto-cervical keratinocyte cells immortalized by HPV16 virus genome. Growth of this cell line is dependent to EGF.
Suggested Medium
MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml BPE. MCDB153 with < 0.5% FCS was also used in original report.
Quality Control
NCE16 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Subculture
Cells are harvested after treatment with 0.1% trypsin and 0.01% EDTA.
Shippment
NCE16 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
NCE16 Cell Line products are for research use only.
