Description
Nb2-11 cell line is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from Glow Biologics on request.
Adherent Suspension
Suspension
Suggested Medium
Fischer's medium (Life Technologies product number 21475025) + 10%Foetal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Na Bicarbonate + 0.05mM 2-Mercaptoethanol (2ME) + 2mM Glutamine.
Quality Control
Nb2-11 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
Nb2-11 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Nb2-11 Cell Line is for research use only
