Description
A microglial cell line from p53-deficient mice.
Suggested Medium
3/7 mixture of DMEM (high glucose) with 10% FBS and A1(IFO 50519)-conditioned medium (CM). The CM is filtered (0.22 um) supernatant of subconfluent A1 culture.
Quality Control
MG5 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
microglia-like
Subculture
Use bacteria-grade dish/flask. For passage, wash the culture twice with PBS and detach cells in PBS by vigorous pipetting with Pasteur pipette.
Shippment
MG5 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MG5 Cell Line products are for research use only.
