Description
Primary mouse embryo fibroblast (MEF) cultures from Ogg1 mutant mice were established by standard procedures from individual embryonic day 13.5 (E13.5) embryos derived from heterozygous matings. Cells were cultured in DMEM/Ham's F-12 (31) with 10% FBS and genotyped by Southern hybridization. Permanent cell lines were established from transformed clones arising spontaneously after repeated passage in culture.
Adherent Suspension
Adherent
Suggested Medium
DMEM/Ham's F-12 (31) with 10% FBS
Quality Control
MEF OGG1 KO Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
MEF OGG1 KO Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MEF OGG1 KO Cell Line is for research use only
