Description
The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 and PEA13, which express heterozygous and homozygous forms of LPR, an experimental system cell line is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
MEF-1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Fibroblast-like
Shippment
MEF-1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MEF-1 Cell Line is for research use only
