Description
MDCK cell line adapted to grow in the protein-free medium 'MDCK Protein-free Medium' (Sigma 14581C). Stirred culture (or Ultra low attachment flasks Corning 3814) is recommended, cells grow in suspended clumps.
Adherent Suspension
Aggregates in suspension
Suggested Medium
MDCK Protein-free medium (Sigma 14581C) + 3.3mM Glutamine. When freezing cells down use Cell Freezing Medium (Sigma C2639) + 10% DMSO or use 5050 fresh culture mediumconditioned medium plus 10% DMSO.
Quality Control
MDCK-Protein Free Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
MDCK-Protein Free Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MDCK-Protein Free Cell Line is for research use only
