Description
MDCK I - Madin-Darby canine kidney- cell line is a subclone derived from the heterogeneous parent line MDCK. MDCK-I cells, also known as "low passage MDCK" are reported to display a high electrical resistance and form a monolayer with characteristics of tight epithelia of the renal collecting duct. Grown on filters, the cells show a cuboidal polarised morphology and express microvillae-like projections on the apical surface. Cultures on glass possess junctions but no polarised or columnar morphology. MDCK-1 cells display an adrenalin, vasopressin and prostoglandin E sensitive adenyl cyclase. Please note, these cells were mycoplasma erradicated prior to deposit at Glow Biologics. Epithelial phenotype may be unstable. Incomplete harvesting at confluence may select for smooth-muscle phenotype this is important if cells being used for transepithelial resistance studies.
Adherent Suspension
Adherent
Suggested Medium
MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
MDCK I Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polarised epithelial
Shippment
MDCK I Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MDCK I Cell Line is for research use only
