Description
The MCF7/LetR-1 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors.Letrozole-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10-7 M testosterone. A culture of MCF-7 cells were treated with 10-6M letrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with letrozole. After ~2-3 months, the isolated colonies gave rise to letrozole-resistant cell lines, which could be grown in letrozole-containing medium with a weekly split ratio of ~125. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.
Adherent Suspension
Adherent
Suggested Medium
Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 1 uM letrozole
Quality Control
MCF7/LetR-1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polygonal epithelia
Shippment
MCF7/LetR-1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MCF7/LetR-1 Cell Line is for research use only
