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MCF7/AnaR-4 Cell Line

Cat.No.
GBTC-2703E
Description
The MCF7/AnaR-4 cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. This breast cancer cell line resistant to the aromatase inhibitor anastrozole and is oestrogen receptor positive.Derivation Anastrozole-resistant cell lines were established from MCF7 cells grown in medium with 10% newborn calf serum (NCS) and 10−7 Molar testosterone. A culture of MCF7 cells were treated with 10−7 Molar anastrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with anastrozole. After ~2-3 months, the isolated colonies gave rise to anastrozole-resistant cell lines, which could be grown in anastrozole-containing medium with a weekly split ratio of ~125. The MCF7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF7 cell line
Biosafety Level
1
Adherent Suspension
Adherent
Size
1x10^6 cells/vial
Suggested Medium
Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum (NCS), 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM anastrozole
Quality Control
MCF7/AnaR-4 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Breast
Morphology
Polygonal
Shippment
MCF7/AnaR-4 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MCF7/AnaR-4 Cell Line is for research use only

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