Description
The MCF7/182R-6 Cell line cell line is a breast cancer cell line resistant to fulvestrant. The MCF7/182R-6 cell line has been established from a clone of MCF7/S0.5 cells surviving long term growth with the pure steroidal antiestrogen ICI 182,780 in 100 nM concentration, see Lykkesfeldt et al (1995). The MCF7/182R-6 cells are grown in the presence of fulvestrant.
Adherent Suspension
Adherent
Suggested Medium
Phenol red free DMEM/F12 (11) supplemented with 1% FCS, Glutamax 2.5 mM and 6 ng/ml insulin. Supplemented with 100nM fulvestrant to maintain resistance.
Quality Control
MCF7/182R-6 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polygonal epithelia
Shippment
MCF7/182R-6 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
MCF7/182R-6 Cell Line is for research use only
