Description
Mbd3KO stem cells have been genetically modified to produce a null phenotype for Mbd3, a critical determinant for lineage commitment in embryonic stem cells. This lack of Mbd3 (Mbd3 exons 2-7 have been replaced by an IRES-?geo-pA sequence) allows them to be maintained in the absence of any exogenous factors and they fail to commit to developmental lineages. Upon re-introduction of the Mbd3 gene the cells are able to differentiate into identifiable bone, muscle, skin, fat and neuronal tissue. Mbd3 null embryonic stem cells can be used to study LIF-independent self-renewal to develop human stem cell lines and new cell culture media. They can also be used to develop specific stem cell lines for high throughput screening of therapeutic compounds. Finally they can be used to develop transgenics for the study of specific diseases or for the development of specific therapeutic stem cell lines for replacement therapies to treat certain diseases.
Adherent Suspension
Adherent
Suggested Medium
GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM beta-mercaptoethanol + LIF 1000 Units/ml (ESGRO ESG1106). Use of LIF is optional, but its addition reduces differentiation.
Quality Control
Mbd3KO Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Adherent monolayer of small spheroidal cells on gelatine
Shippment
Mbd3KO Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Mbd3KO Cell Line is for research use only
