Description
Established in 1974 from the second in a series of 3 primary colonic tumours resected from a 65 year old Caucasian female, which was classified as a Duke's B mucin producing colon adenocarcinoma. LS 123 cell line cell line is aneuploid, fails to grow progressively in nude mice and forms colonies only in enriched semisolid medium developed for tumour stem cells. The cells will grow in either low serum or serum free medium. In chick embryonic skin assays the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. LS 123 exhibits several properties associated with neoplasia, but have little invasive potential and may represent an early stage in tumour development.
Adherent Suspension
Adherent
Suggested Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat inactivated).
Quality Control
LS 123 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
LS 123 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
LS 123 Cell Line is for research use only
