Description
The parent L strain was derived from normal subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse by WR Earle in 1940. NCTC clone 929 Clone of strain L (also known as L929) was subsequently derived in March 1948. Strain L was one of the first cell strains to be established in continuous culture, and clone 929 was the first cloned strain developed. Clone 929 was established (by the capillary technique for single cell isolation) from the 95th subculture generation of the parent strain. L929S grows spontaneously in suspension in low serum concentrations. It can also be grown in serum-free medium supplemented with 0.5% bactopeptone.
Adherent Suspension
Suspension
Suggested Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
L929S Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
L929S Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
L929S Cell Line is for research use only
