Description
The myogenic cell line L8 was isolated by D Yaffe in 1969 by selective serial passage of myoblasts from primary rat skeletal muscle cell cultures (new born, non-in-bred Wistar rats). Subsequently they were recloned by H Blau. L8 cells differentiate into cross-striated myotubes by fusion and express several muscle-specific proteins. The myoblast population will be depleted if cells are allowed to become confluent before sub-culturing. Therefore, sub-cultivation before confluency and periodical re-cloning is necessary. The preparation of a frozen stock at early passage is recommended.
Adherent Suspension
Adherent
Suggested Medium
DMEM Medium 199 (41) + 2mM Glutamine + 1% CEE (Chick Embryo extract (Seralab)) + 10% Horse Serum (HS).
Quality Control
L8 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
L8 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
L8 Cell Line is for research use only
