Description
Derived from Jurkat E6-1 clone after gamma irradiation and complement-mediated lysis with anti CD45. Low expression of CD45 (5-8% compared to parent line) results in defective signal transduction through TcR and the accessory molecule, CD2. Interleukin 2 (IL-2) is produced in response to calcium ionophore and phorbol ester stimulation, but it does not produce IL-2 in response to TcR or CD2 stimulation.
Adherent Suspension
Suspension
Suggested Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
J45.01 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
J45.01 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
J45.01 Cell Line is for research use only
